Coding

Part:BBa_K1916012

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-11)


CBCR3 + intein-CBD tag

Fusion protein consisting of the GAF domain of an uncharacterized cyanobacteriochrome (CBCR) sequence and an intein/chitin-binding domain attached to the C-terminus for purification. Labelled as CBCR3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 513
    Illegal SpeI site found at 540
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 540
    Illegal NgoMIV site found at 1036
    Illegal AgeI site found at 1126
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None